Serial serum creatine phosphokinase MB isoenzyme activity after myocardial infarction. Studies in the baboon and man.

Diagnosis of myocardial infarction by serum isoenzyme analysis.

Serial monitoring of the serum isoenzyme patterns of creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) in patients suspected of acute myocardial infarction has become a highly sensitive and specific diagnostic method. The predictable evolution of isoenzyme patterns following infarction permits diagnosis and recognition of early stages, recovery stages and extension of infarction in t...

Creatine kinase MB isoenzyme studies in diagnosis of myocardial infarction.

Serial measurements of the MB isoenzyme of creatine kinase, total creatine kinase, aspartate mainotransferase, and urea stable lactate dehydrogenase have been made in the serum of a series of 139 patients admitted with a diagnosis of suspected myocardial infarction. Serum MB isoenzyme measurements have also been made on postoperative surgical patients and patients with medical disorders known t...

Kinetic properties of the isoenzymes of human creatine phosphokinase.

Studies of the three human creatine phosphokinase (EC 2.7.3.2) isoenzymes, MM, MB, and BB, show that important differences exist in substrate dependency of the reaction rates. A method was developed to study these properties in which the ATP formed in the reverse reaction was measured by means of firefly luciferase. With substrate conditions at which the isoenzymes showed substantial difference...

Assessment of The Diagnostic Capability of Serum Creatine Phosphokinase and Its Isoenzyme in Ectopic Pregnancy

Cardiac specific creatine phosphokinase isoenzyme in the diagnosis of acute myocardial infarction.

The specific and sensitivity of serum creatine phosphokinase cardiac specific isoenzyme (MB) in the diagnosis of acute myocardial infarction (AMI) was evaluated. An ion-exchange chromatographic technique was used to isolate MB. Sera layered on mini-columns of DEAE-Sephadex were eluted with Tris-buffered sodium chloride. Quantification of isolated MB was performed by creatine phosphokinase (CPK)...